BrightLine® Single-edge Laser Dichroic Beamsplitters

   

These 45-degree angle of incidence dichroic beamsplitters are optimized for the most popular lasers used for  fluorescence imaging, including newer all-solid-state lasers. Reflection is guaranteed > 98% (s-polarization) and > 94% (average polarization) at the laser wavelengths, plus > 93% average transmission and very low ripple over extremely wide passbands – out to 900 and even 1200 nm. The transition from reflection to transmission is exceptionally steep (< 2.5% of the longest laser wavelength) to maximize capture of weak signals close to the laser line – making these a perfect match to the normal incidence long-wave-pass EdgeBasic™ filters.

BrightLine Laser Dichroics are designed to contribute less than one Rayleigh Range of shift in focus (relative to a perfectly flat mirror) at the focal plane of a microscope objective or other focusing lens after reflecting a collimated Gaussian laser beam with a waist diameter up to 2.5 mm.

Applications include:
   • Any microscope or imaging system that benefits from premium beamsplitter spectral performance and/or flatness
   • Laser-scanning confocal microscopes (e.g. Olympus FluoView FV300, FV1000; Nikon Eclipse C1 Plus/si, A1)
   • Spinning-disk confocal microscopes (e.g. BD Biosciences CARV II; Olympus DSU)
   • Total-internal-reflection fluorescence (TIRF) microscopes (e.g. Olympus TIRF Illuminator, FV1000-EVA; Nikon TIRF)

   • Structured illumination microscopes (e.g. Qioptiq OptiGrid®)
These rectangular 25 mm x 36 mm x 1 mm thick dichroics fit popular filter "cubes" for fluorescence microscopes from Nikon, Olympus and Zeiss (contact Semrock for sizes for Leica microscopes).  Same-day shipping available for most of these products, or custom-sizing in less than a week!
How Laser Dichroics fit into the Semrock filter line-up: These 45-degree beamsplitters can be mixed and matched with a wide variety of bandpass filters and long-pass filters, and are included in our Laser Fluorescence sets.  Semrock also stocks a wide variety of other single-edge dichroicsimaging dichroics, and multiedge dichroic beamsplitters.  Semrock's custom-fabricated Beamsplitter Mount is ideal for holding beamsplitters in laboratory bench-top setups.

Edge ColorPart NumberLaser
Wavelengths (nm)
Reflection
Band (nm)
Nominal Edge
Wavelength (nm)
Transmission
Band (nm)
Price1
Di01-R405-25x36 375 +/- 3
405 +/- 5
372 - 410415420.3 - 900$345
Di01-R442-25x36 440 +3/-1
442.0
457.9
439 - 457.9463.5469.3 - 900$345
Di01-R488-25x36 473 +/- 2
488 +3/-2
471 - 491497503.3 - 900$345
Di01-R514-25x36 505.0
514.5
515.0
505 - 515521.5527.9 - 900$345
Di01-R532-25x36 514.5
532
514 - 532542545.3 - 1200$345
Di01-R561-25x36 559 +/- 5
561.4
568.2
554 - 568.2575582.4 - 1200$345
Di01-R594-25x36 593.5
594.1
594.0 +/- 0.3
593.5 - 594.3605609.2 - 1200$345
Di01-R635-25x36 632.8
635 +7/-0
647.1
632.8 - 647.1654663.3 - 1200$345

1 Prices listed are for US domestic orders only. If you are ordering from outside the US, please contact your nearest regional distributor for the applicable list price.

Wondering which dichroic to use with your fluorophore?

  Laser Wavelengths (nm) 

Part Number

Ideal Fluorophores

375 +/- 3
405 +/- 5

 Di01-R405-25x36

 DAPI or BFP

440 +3/-1
442.0
457.9

 Di01-R442-25x36

 CFP

473 +/- 2
488 +3/-2

 Di01-R488-25x36

 GFP or FITC

505.0
514.5
515.0

 Di01-R514-25x36

 YFP

559 +/- 5
561.4
568.2

 Di01-R561-25x36

 mCherry or TexasRed®

632.8
635 +7/-0
647.1

 Di01-R635-25x36

 Cy5™


Semrock Filters are RoHS Compliant